Precision Agriculture Science and Technology ISSN:2672-0086(Print) 2713-5632(Online) 정밀농업과학기술

Plants when exposed to stressful conditions, accumulates array of metabolites. Among them proline plays significant and beneficial role as osmolyte, metal chelator and as anti-oxidative defense and regulator molecule. Thus the proline content is one of the potent bio-marker for the early detection of abiotic stress in plant. In this work a microfluidic paper-based electrochemical device (µPED) modified with copper oxide nanoparticles (CuO) decorated reduced graphene oxide (rGO) nanocomposite is utilized to develop disposable system for the detection of proline. Wax and screen printings were utilized to create microfluidic chambers and electrode pattern on paper device, respectively. Portable potentiostat was used for measuring the current in response from the addition of proline. The proposed sensor exhibits excellent electrochemical performance with broad linear responses over a proline concentration ranging from 0.1 mM to 7.5 mM (R2 =0.99) and showed lower detection limit of 0.05 mM at 0.4 V vs. pseudo Ag/AgCl. Excellent electrochemical performance may be attributed to presence of electroactive rGO and catalytic activity towards proline is due to the coordination between proline and copper (II) oxide nanoparticles. These results demonstrate that the proposed paper-based electrochemical sensor can be utilized in disposable and portable way to detect proline content in the abiotic-stressed plants and has potential to be applied in on-site application.