All Issue

2024 Vol.6, Issue 3

Research Article

Colorimetric sensing platform based on Lab-on-a-chip for ammonia detection in water
JooYoung Lee1
,
Yeonggeol Hong1
,
Kyoung-Je Jang12*
1Department of Bio-Systems Engineering, Institute of Smart Farm, Gyeongsang National University, Jinju 52828, Republic of Korea
2Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea
*Corresponding Author
30 September 2024. pp. 165-178
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Abstract

Ammonia, a compound of nitrogen and hydrogen, is highly corrosive, toxic, and harmful to living organisms when exposed to high concentrations. It is also a known cause of water pollution when released in large quantities. This common source of ammonia in agriculture and factories requires continuous monitoring and rapid field measurements to prevent water pollution. Various methods exist for monitoring ammonia, with electrochemical and optical methods being the primary ones. Conventional methods face challenges such as sensor longevity, size, and low detection limits. In this study, lab on a chip module was fabricated for ammonia measurement. The developed device comprises three distinct parts: a mixing part, a reaction part, and a measuring part. It is designed to be compatible with microfluidic chips, allowing for the continuous utilization of the platform. The replacement of the microfluidic chip is the only maintenance required, making the device cost-effective for ammonia measurement in water. The ammonia concentration was measured using a colorimetric detection method on the lab-on-a-chip detection unit, using a 20% v/v dilution of Nessler's reagent and the reaction product of the ammonia sample with the AS 7262 spectral sensor. To facilitate straightforward measurements on the developed platform, 3D computer-aided design software was used to create a digital model, which was then produced using a 3D printer employing the 3D modelling technique.

Keywords
Ion sensing
Microfluidic chip
Nessler’s reagent
PDMS
3D printing
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Information
  • Publisher :Korean Society of Precision Agriculture
  • Publisher(Ko) :한국정밀농업학회
  • Journal Title :Precision Agriculture Science and Technology
  • Journal Title(Ko) :정밀농업과학기술
  • Volume : 6
  • No :3
  • Pages :165-178
  • Received Date : 2024-07-02
  • Revised Date : 2024-08-27
  • Accepted Date : 2024-08-27
  • DOI :https://doi.org/10.22765/pastj.20240012
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